Annett Urbanek

Following my training as a medical technologist for laboratory analysis (MTL) and further training in the field of molecular biology, I gained experience in various research areas and acquired a wide range of skills in numerous laboratory techniques in the fields of mass spectrometry, cell- and bacteria culture, proteomics, genetic engineering and histological tissue processing.
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My focus is on supporting laboratory research work and optimizing laboratory conditions for an ideal research environment in the laboratory to offer scientists the best possible space for new ideas and approaches.
Patent list
  • PCT-Patent pending:
Urbanek, A. (2024) Modulares Laborkühlschrankaufbewahrungssystem. PCT/DE2024/101058.
  • Registered design:
Urbanek, A. (2023) Aufbewahrungsbehälter für Kühlschränke. DE 402023100879.4 
https://register.dpma.de/DPMAregister/gsm/register?DNR=402023100879-0001
  • Registered utility model:
Urbanek, A. (2023) Modulares Aufbewahrungssystem für Laborkühlschränke. DE202023107327U1.
https://register.dpma.de/DPMAregister/pat/register?AKZ=2020231073271

Publication list

Note: Publications are published under both the current name [Annett Urbanek] or the maiden name [Annett Bleul].
 
  • Mandal, S., Motganhalli Ravikumar, R., Tannert, A., Urbanek, A., Guliev, R. R., Naumann, M., ... & Neugebauer, U. (2025). Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis. Scientific Reports, 15(1), 1153. www.nature.com/articles/s41598-024-84330-2
  • Greiner, S., Ebrahimi, M., Rodewald, M., Urbanek, A., Meyer-Zedler, T., Schmitt, M., ... & Popp, J. (2024). Characterizing Metabolic Shifts in Septic Murine Kidney Tissue Using 2P-FLIM for Early Sepsis Detection. https://www.mdpi.com/2306-5354/12/2/170
  • Bottek, J., Soun, C., Lill, J. K., Dixit, A., Thiebes, S., Beerlage, A. L., Horstmann, M., Urbanek, A.,...von Eggeling, F. & Engel, D. R. (2020). Spatial proteomics revealed a CX3CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder. Mucosal Immunology, 13(4), 702-714. https://www.nature.com/articles/s41385-020-0269-7
  • Bottek, J., Soun, C., Volke, J. K., Dixit, A., Thiebes, S., Beerlage, A. L., Horstmann, M., Urbanek, A.,...von Eggeling, F. & Engel, D. R. (2019). Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection. bioRxiv, 649137. https://www.biorxiv.org/content/10.1101/649137v1
  • Urbanek, A., Hölzer, S., Knop, K., Schubert, U. S., & von Eggeling, F. (2016). Multigrid MALDI mass spectrometry imaging (mMALDI MSI). Analytical and Bioanalytical Chemistry, 408, 3769-3781. https://link.springer.com/article/10.1007/s00216-016-9465-4
  • Bocklitz, T., Bräutigam, K., Urbanek, A., Hoffmann, F., von Eggeling, F., Ernst, G., ... & Popp, J. (2015). Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies. Analytical and bioanalytical chemistry, 407, 7865-7873. https://link.springer.com/article/10.1007/s00216-015-8987-5
  • Kaufmann, R., Mußbach, F., Urbanek, A., Settmacher, U., & von Eggeling, F. (2014). Proteomic Effects of the Coagulation Proteinase Thrombin on LX-2 Hepatic Stellate Cells. Journal of Medical Biochemistry, 33(4), 371.
  • Knop, K., Pretzel, D., Urbanek, A., Rudolph, T., Scharf, D. H., Schallon, A., ... & Schubert, U. S. (2013). Star-shaped drug carriers for doxorubicin with POEGMA and POEtOxMA brush-like shells: a structural, physical, and biological comparison. Biomacromolecules, 14(8), 2536-2548.
  • Ruediger, T., Berg, A., Guellmar, A., Rode, C., Schnabelrauch, M., Urbanek, A., ... & Sigusch, B. W. (2012). Cytocompatibility of polymer-based periodontal bone substitutes in gingival fibroblast and MC3T3 osteoblast cell cultures. Dental Materials, 28(10), e239-e249.
  • Delaney, J. T., Urbanek, A., Wehder, L., Perelaer, J., Crecelius, A. C., von Eggeling, F., & Schubert, U. S. (2011). Combinatorial optimization of multiple MALDI matrices on a single tissue sample using inkjet printing. ACS Combinatorial Science, 13(3), 218-222.
  • Von Eggeling, F., Bleul, A., Loncarevic, I. F., Michel, S., Heller, A., & Melle, C. (2008). Tapping an unexploited repository: Carnoy's fixed cell pellets for proteomic biomarker research in leukemia. Molecular Medicine Reports, 1(4), 585-587.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., & von Eggeling, F. (2008). Colon-derived liver metastasis, colorectal carcinoma, and hepatocellular carcinoma can be discriminated by the Ca2+-binding proteins S100A6 and S100A11. PloS one, 3(12), e3767.
  • Scholl, S., Melle, C., Bleul, A., Spies-Weisshart, B., Kunert, C., Höffken, K., & Von Eggeling, F. (2007). Specific pattern of protein expression in acute myeloid leukemia harboring FLT3-ITD mutations. Leukemia & lymphoma, 48(12), 2418-2423.
  • Driemel, O., Murzik, U., Escher, N., Melle, C., Bleul, A., Dahse, R., ... & von Eggeling, F. (2007). Protein profiling of oral brush biopsies: S100A8 and S100A9 can differentiate between normal, premalignant, and tumor cells. PROTEOMICS–Clinical Applications, 1(5), 486-493.
  • Melle, C., Ernst, G., Scheibner, O., Kaufmann, R., Schimmel, B., Bleul, A., ... & von Eggeling, F. (2007). Identification of specific protein markers in microdissected hepatocellular carcinoma. Journal of proteome research, 6(1), 306-315. 
  • Melle, C., Ernst, G., Escher, N., Hartmann, D., Schimmel, B., Bleul, A., ... & von Eggeling, F. (2007). Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker. Clinical chemistry, 53(4), 629-635.
  • Ernst, G., Melle, C., Schimmel, B., Bleul, A., & von Eggeling, F. (2006). Proteohistography–direct analysis of tissue with high sensitivity and high spatial resolution using ProteinChip technology. Journal of Histochemistry & Cytochemistry, 54(1), 13-17. 
  • Escher, N., Spies-Weißhart, B., Kaatz, M., Melle, C., Bleul, A., Driesch, D., ... & von Eggeling, F. (2006). Identification of HNP3 as a tumour marker in CD4+ and CD4− lymphocytes of patients with cutaneous T-cell lymphoma. European Journal of Cancer, 42(2), 249-255.
  • Melle, C., Bogumil, R., Ernst, G., Schimmel, B., Bleul, A., & von Eggeling, F. (2006). Detection and identification of heat shock protein 10 as a biomarker in colorectal cancer by protein profiling. Proteomics, 6(8), 2600-2608.
  • Kriegova, E., Melle, C., Kolek, V., Hutyrova, B., Mrazek, F., Bleul, A., ... & Petrek, M. (2006). Protein profiles of bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis. American journal of respiratory and critical care medicine, 173(10), 1145-1154.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., Mothes, H., Kaufmann, R., ... & Von Eggeling, F. (2006). Different expression of calgizzarin (S100A11) in normal colonic epithelium, adenoma and colorectal carcinoma. International Journal of Oncology, 28(1), 195-200.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., Thieme, H., Kaufmann, R., ... & Von Eggeling, F. (2005). Discovery and identification of α-defensins as low abundant, tumor-derived serum markers in colorectal cancer. Gastroenterology, 129(1), 66-73.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., Kaufmann, R., Hommann, M., ... & von Eggeling, F. (2005). Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach. Journal of proteome research, 4(5), 1799-1804.
  • Melle, C., Osterloh, D., Ernst, G., Schimmel, B., Bleul, A., & Von Eggeling, F. (2005). Identification of proteins from colorectal cancer tissue by two-dimensional gel electrophoresis and SELDI mass spectrometry. International Journal of Molecular Medicine, 16(1), 11-17.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., Koscielny, S., Wiesner, A., ... & von Eggeling, F. (2004). A technical triade for proteomic identification and characterization of cancer biomarkers. Cancer research, 64(12), 4099-4104.
  • Melle, C., Kaufmann, R., Hommann, M., Bleul, A., Driesch, D., Ernst, G., & Von Eggeling, F. (2004). Proteomic profiling in microdissected hepatocellular carcinoma tissue using ProteinChip® technology. International journal of oncology, 24(4), 885-891.
  • Melle, C., Ernst, G., Schimmel, B., Bleul, A., Koscielny, S., Wiesner, A., ... & von Eggeling, F. (2003). Biomarker discovery and identification in laser microdissected head and neck squamous cell carcinoma with ProteinChip® technology, two-dimensional gel electrophoresis, tandem mass spectrometry, and immunohistochemistry. Molecular & Cellular Proteomics, 2(7), 443-452.

CV

Education:

1998-2001:          Medizinische Technologin für Laboratoriumsanalytik (MTL) at SBBS für Gesundheit und Soziales, Jena
2006:                   Fachkraft für Molekularbiologie (Gläsernes Labor Berlin)
 
Professional experience:

2001:                    Clinical diagnostics laboratory of Landesfachkrankenhaus Stadtroda,
2002-2009          Institute of Human Genetics, University Hospital Jena
2009-2010          Research Laboratory of Clinic for Conservative Dentistry, University Hospital Jena
2010-2016           Institute for Organic and Macromolecular Chemistry, University Jena
Since 2016 -        Leibniz Institute of Photonic Technology, Jena, Jena Biophotonic and Imaging Laboratory /(JBIL), Clinical Spectroscopic Diagnostics Department, Microscopy Department, Biophysical Imaging Department.