Agnes Koerfer

I started my PhD with Christian Eggeling after finishing my studies in physics in 2019. The main goal of my project is establishing novel MINFLUX super-resolution microscopy for quantitative imaging with environmental sensors like solvatochromic dyes. I am also working on an open-source analysis pipeline for MINFLUX tracking experiments that segments the tracks based on a confocal image. With this experimental procedure, I can differentiate molecules inside or outside of virus budding sites. I use these sensors, as well as fluorescent lipid analogues, to study the dynamic membrane organization during HIV assembly on the plasma membrane of live cells.

Publication list

• Dasgupta, A., Koerfer, A., Kokot, B., Urbanèiè, I., Eggeling, C., & Carravilla, P. (2024). Effects and avoidance of photoconversion-induced artifacts in confocal and STED microscopy. Nature Methods, 1-4.
• ​ A. Koerfer, F. Reina, C. Eggeling. Influence of different surface cleaning methods on STED-FCS and scanning STED-FCS calibration measurements. J Microsc DOI: 10.1111/jmi.13250 (2023)

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CV

• 2019 - ... :     PhD. Student in Christian Eggeling's Lab
• 2013 - 2019: Diplom-Physicist, Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau, Germany