Jonatan AlvelidI am an engineering physicist by training, and did my PhD in the lab of Ilaria Testa working on automating STED microscopy for functional and structural live-cell imaging. The thesis included development of a STED microscope, and work on microscope control software and automation, image analysis, fluorophores, and various applications. I am currently interested in and focusing on microscopy automation and image analysis, and developing tools to support more advanced use of super-resolution microscopes for biological applications. I work on applying smart microscopy automation and data analysis to MINFLUX microscopy, both for imaging and tracking. In that I aim to let the sample tell us when, where, and how to acquire, thereby allowing more efficient data collection and experiments that are not feasible with manual control.
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Publication list
CV
- Hirabayashi, Y., Lewis, T.L.J., Du, Y., Virga, D.M., Decker, A.M., Coceano, G., Alvelid, J., Paul, M.A., Hamilton, S., Kneis, P., Takahashi, Y., Gaublomme, J.T., Testa, I., Polleux, F., Most axonal mitochondria in cortical pyramidal neurons lack mitochondrial DNA and consume ATP, biorXiv, doi.org/10.1101/2024.02.12.579972 (2024).
- Pennacchietti, F., Alvelid, J., Morales, R.A., Damenti, M., Ollech, D., Oliinyk, O.S., Shcherbakova, D.M., Villablanca, E.J., Verkhusha, V.V., Testa, I., Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms, Nature Communications, doi.org/10.1038/s41467-023-44054-9 (2023).
- Volpato, A., Ollech, D., Alvelid, J., Damenti, M., Müller, B., York, A.G., Ingaramo, M., Testa, I., Extending fluorescence anisotropy to large complexes using reversibly switchable proteins, Nature Biotechnology, doi.org/10.1038/s41587-022-01489-7 (2023).
- Alvelid, J., Bucci, A., Testa, I., Far Red-Shifted CdTe Quantum Dots for Multicolour Stimulated Emission Depletion Nanoscopy, ChemPhysChem, doi.org/10.1002/cphc.202200698 (2023).
- Alvelid, J., Damenti, M., Sgattoni, C., Testa, I., Event-triggered STED imaging, Nature Methods, doi.org/10.1038/s41592-022-01588-y (2022).
- Jalalvand, E., Alvelid, J., Coceano, G., Edwards, S., Robertson, B., Grillner, S., Testa, I., ExSTED microscopy reveals contrasting functions of dopamine and somatostatin CSF-c neurons along the lamprey central canal, Elife, doi.org/10.7554/eLife.73114 (2022).
- Casas Moreno, X., Al-Kadhimi, S., Alvelid, J., Bodén, A., Testa, I., ImSwitch: Generalizing microscope control in Python, Journal of Open Source Software, doi.org/10.21105/joss.03394 (2021).
- Fang, T., Alvelid, J., Spratt, J., Ambrosetti, E., Testa, I., Teixeira, A.I., Spatial Regulation of T-Cell Signaling by Programmed Death-Ligand 1 on Wireframe DNA Origami Flat Sheets, ACS Nano, doi.org/10.1021/acsnano.0c10632 (2021).
- Alvelid, J. and Testa, I., Stable stimulated emission depletion imaging of extended sample regions, Journal of Physics D: Applied Physics, doi.org/10.1088/1361-6463/ab4c13 (2020).
- Matlashov, M.E.*, Shcherbakova, D.M.*, Alvelid, J.*, Baloban, M., Pennacchietti, F., Shemetov, A.A., Testa, I., Verkhusha, V.V., A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales, Nature Communications, doi.org/10.1038/s41467-019-13897-6(2020).
- Alvelid, J. and Testa, I., Fluorescence microscopy at the molecular scale, Current Opinion in Biomedical Engineering, doi.org/10.1016/j.cobme.2019.09.009(2019)-
- Schramm, F.D., Schroeder, K., Alvelid, J., Testa, I., Jonas, K., Growth-driven displacement of protein aggregates along cell length ensures partitioning to both daughter cells in Caulobacter crescentus, Molecular Microbiology, doi.org/10.1111/mmi.14228 (2019).
CV
- 2023- Postdoc, Laboratory of Christian Eggeling, Department of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany
- 2021-2023 Postdoc, Laboratory of Ilaria Testa, Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
- 2016–2021 PhD, Laboratory of Ilaria Testa, Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden
- 2014–2016 Master of Science in Nanotechnology, KTH Royal Institute of Technology, Stockholm, Sweden
- 2011-2014 Bachelor of Science in Engineering Physics, KTH Royal Institute of Technology, Stockholm, Sweden