Dr. Katharina Reglinski

I am interested in the mechanisms and dynamics of peroxisomes and protein import into peroxisomes. As peroxisomes are small organelles, I apply (live) STED microscopy to investigate spatial organization and dynamics of peroxisomal proteins. This can reveal new understandings on processes like translocation of proteins across the peroxisomal membrane.

Publication list

• ​ A. Seltmann, P. Carravilla, K. Reglinski, C. Eggeling*, D. Waithe*. Neural network informed photon filtering reduces fluorescence correlation spectroscopy artifacts. Biophys J (2024).
  
• J. Joseph, L. Spantzel, M. Ali, D. Moonnukandathil Joseph, S. Unger, K. Reglinski, C. Krafft, A. D. Muller, C. Eggeling, R. Heintzmann, M. Borsch, A. T. Press, D. Tauber. Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR). Spectrochim Acta A Mol Biomol Spectrosc 306, 123612 (2024).
• ​C. M. Svensson, K. Reglinski, W. Schliebs, R. Erdmann, C. Eggeling*, M. T. Figge. Quantitative analysis of peroxisome tracks using a Hidden Markov Model. Sci Rep 13, 19694 (2023).
• Galiani, Silvia, Eggeling, Christian and Reglinski, Katharina. "Super-resolution microscopy and studies of peroxisomes" Biological Chemistry, vol. 404, no. 2-3, 2023, pp. 87-106. https://doi.org/10.1515/hsz-2022-0314
• ​Ghosh, Mausumi, Denkert, Niels, Reuter, Maren, Klümper, Jessica, Reglinski, Katharina, Peschel, Rebecca, Schliebs, Wolfgang, Erdmann, Ralf and Meinecke, Michael. "Dynamics of the translocation pore of the human peroxisomal protein import machinery" Biological Chemistry, vol. 404, no. 2-3, 2023, pp. 169-178. https://doi.org/10.1515/hsz-2022-0170
• Galiani S, Reglinski K, Carravilla P, Barbotin A, Urbančič I, Ott J, Sehr J, Sezgin E, Schneider F, Waithe D, Hublitz P, Schliebs W, Erdmann R, Eggeling C. Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5. Biophys Rep. 2022;2(2) doi: 10.1016/j.bpr.2022.100055​
• Reglinski K, Steinfort-Effelsberg L, Sezgin E, Klose C, Platta HW, Girzalsky W, Eggeling C, Erdmann R. Fluidity and Lipid Composition of Membranes of Peroxisomes, Mitochondria and the ER From Oleic Acid-Induced Saccharomyces cerevisiae. Front Cell Dev Biol. 2020;8:574363.
• Büttner M, Lagerholm CB, Waithe D, Galiani S, Schliebs W, Erdmann R, Eggeling C, Reglinski K. Challenges of using Expansion Microscopy for super-resolved imaging of cellular organelles. Chembiochem. doi: 10.1002/cbic.202000571.
• Waithe D, Brown JM, Reglinski K, Diez-Sevilla I, Roberts D, Eggeling C. Object detection networks and augmented reality for cellular detection in fluorescence microscopy. J Cell Biol. 2020;219(10):e201903166.
• Schneider KRA, Chettri A, Cole H, et al. Intracellular Photophysics of an Osmium Complex bearing an Oligothiophene Extended Ligand. Chemistry. 2020;10.1002/chem.202002667.
• Blayney J*, Foster EM*, Jagielowicz M*, Kreuzer M*, Morotti M*, Reglinski K*, Xiao JH*, Hublitz P. Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions. Methods Protoc. 2020, 3, 53. doi: 10.3390/mps3030053
• Xiong Y, Vargas Jentzsch A, Osterrieth JWM, et al. Spironaphthoxazine switchable dyes for biological imaging. Chem Sci. 2018;9(11):3029-3040. doi:10.1039/c8sc00130h
• Galiani S, Waithe D, Reglinski K, et al. Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins. J Biol Chem. 2016;291(33):16948-16962. doi:10.1074/jbc.M116.734038
• Reglinski K, Keil M, Altendorf S, et al. Peroxisomal Import Reduces the Proapoptotic Activity of Deubiquitinating Enzyme USP2. PLoS One. 2015;10(10):e0140685. doi:10.1371/journal.pone.0140685
• Fodor K, Wolf J, Reglinski K, et al. Ligand-induced compaction of the PEX5 receptor-binding cavity impacts protein import efficiency into peroxisomes. Traffic. 2015;16(1):85-98. doi:10.1111/tra.12238

*Equal contribution

CV

• 2005-2010 Studies of Biology and Biotechnology (Diploma), Ruhr-University Bochum, Germany
• 2010-2014 PhD, Institute of Physiological Chemistry, Systemic Biochemistry, Ruhr-University Bochum, Germany
• 2015 – 2019, PostDoc, Christian Eggeling Laboratory, Weatherall Institute of Molecular Medicine, University of Oxford, UK.
• Since April 2019 Postdoc, Eggeling lab, Institute of Applied Optics and Biophysics, Fredrich-Schiller University, Jena